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政府計畫
計畫名稱(中文) 分子標誌及組培技術在育種、品種及種子純度鑑定等技術開發利用
計畫名稱(英文) Development of molecular markers and tissue culture techniques on breeding, seed purity and variety identification.
文獻分類 其它
計畫編號 106農科-8.6.3-子-X1
計畫主辦人 鍾文全
計畫主管機關 行政院農業委員會
計畫執行機構 行政院農業委員會種苗改良繁殖場
全程計畫年 2017年 ~ 2017年
中文關鍵字 病原性;綠瑪娣雜交種;細定位;米飯光澤度;屬內雜交;辣椒榕;複合式聚合酶連鎖反應;夜來香微嵌紋病毒;增殖;屬間雜交;萬代蘭類;表現序列標幟;分生組織;組織培養;矮南瓜黃化嵌紋病毒;簡單重複序列;多肉植物;標準型雜交種;植物生長調節劑;器官再生;未成熟胚;芋頭;絲瓜;量化繁殖;水草;南瓜;無病毒苗;食味品質;組織培養;光照;亞油酸;逆轉錄核酸聚合酵素連鎖反應法;油酸;頸腐根腐病;紅瑪娣雜交種;抗病篩選;分子標誌;番茄;種子純度檢測;花芽;芽體增殖;中藥;芽體誘導;頸腐根腐病原菌;十字花科;小榕;食用竹類;花
英文關鍵字 Pathogenicity;green Maudiae-type hybrid;Fine genetic mapping;Glossiness of cooked rice;intrageneric hybridization;Bucephalandra sp.;Multiplex PCR;Tuberose mild mosaic virus;multiplication;Intergeneric hybridization;Vanda alliances;Expressed Sequence Tag;m
中文摘要 植物組織培養技術開發及其應用 ■萬代蘭組培技術開發及量產困難蝴蝶蘭芽體誘導生根之探討 本研究目的在於開發新興熱帶蘭花如萬代蘭之組織培養種苗量產技術,並擬提升蝴蝶蘭種苗量產相關技術。利用幼嫩花序或生長點為培植體,探討萬代蘭微體繁殖種苗量產之最適化培養基及培養方式,以期能穩定生產品質良好之瓶苗。某些特定品種不易誘導生根,致生長緩慢或降低種苗整齊度等。本試驗以萬代蘭 Vanda Simirun x Vanda coerulea、V.Pachara Delight等及蝴蝶蘭 Phalaenopsis Chingruey's Goldstaff ‘Gold-1’試管芽體為材料,探討不同培養基組成及LED光源對芽體誘導生根之影響,進而穩定種苗之生長特性與品質之提升。 ■ 芭菲爾鞋蘭屬微體繁殖流程建立及優選雜交單株分生繁殖 芭菲爾鞋蘭引人注目的花色與獨特的花形確實深受消費者喜愛,理應發展出更大的產業規模。由於,多樣化優良品種及確立的微體繁殖系統,儼然為臺灣蝴蝶蘭產業發展的最大動能。因而,臺灣芭菲爾鞋蘭產業的突破性進展,預期亦仰賴新品種之選育及高效率的微體繁殖技術,而這也是臺灣芭菲爾鞋蘭產業發展受限的重大瓶頸。因此,本計畫以建立高效率之芭菲爾鞋蘭微體繁殖系統為目標,同時建立自行育種及養成之新穎優選個體所需的分生繁殖技術。期能有助於臺灣芭菲爾鞋蘭種苗生產量能之提升,以符合國際芭菲爾鞋蘭種苗供應市場之質量需求,更期能促進臺灣芭菲爾鞋蘭產業的整體發展。 ■熱帶觀賞水草健康種苗量產技術之開發 辣椒榕( Bucephalandra sp.)及小榕( Anubias barteri)是水草市場中的高價前景水草,極具發展外銷市場的潛力,然而外銷檢疫及穩定供貨是主要限制因子。本二年期計畫應用植物組織培養技術,開發其健康種苗量產途徑,除可穩定量化生產健康種苗,並可簡化檢疫流程及提升運輸品質。預備試驗中已建立迷你小榕( A. barteri var. nana‘Petite’)的無菌培養,全株經戊二醛除藻2週後,進行表面消毒,以走莖為培植體,培養在含2 mg L -1BA及0.5 mg L -1NAA的1/2 MS基本培養基中進行初代培養,經4週光照培養後,平均每個培植體可增殖13.7個芽體,接續繼代至1/2 MS基本培養基中可正常發根。本計畫預期可經由芽體器官發生,培育繁殖辣椒榕及小榕的健康種苗,未來可開發應用於建立健康種苗繁殖體系,以推動水草產業,促進產業昇級。 ■夜來香優良品種無病毒分生苗快繁與瓶內結球生產體系之開發 本研究擬建構新品種夜來香病毒病害診斷分析、快速檢測、無病毒分生苗快繁及瓶內結球之種球生產體系,從已感染病毒夜來香植株,評估種球莖頂分生組織不同大小對夜來香去病毒暨分生組織培植體(meristem explant)經由體胚發生再生系統之去病毒影響,以建構組培生產健康種苗快繁體系。此外,並於組培量化增殖階段,配合建立之病毒檢測技術,進行健康種苗的精確檢定,期能建立優質的夜來香健康種球生產與檢驗技術。另,研究也將進一步評估瓶內結球技術,以供新品種商業種球生產應用,並整合為夜來香種苗產業開創永續經營之重要技術。 ■瓦葦屬多肉植物之組培量產研發 本計劃將研發多肉植物組織培養的技術,建立多肉植物實生苗及分生苗繁殖體系。利用不同成熟度之葉片、種子、側芽等再生能力強之部位作消毒無菌化,找出最適當的營養系無菌化材料及最佳再生條件,並配合不同濃度賀爾蒙組合,誘導產生器官發生或是胚胎發生,使之分化發育成為完整植株,並將此結果應用在多肉植物的再生繁殖上,以提高此植物的繁殖效率,扶持國內專業的多肉植物業者,建立其組織培養量產的技術平台,以滿足逐漸成長的國內外市場並增加農民之收益。 ZYMV病原性分化對分子標誌篩選抗病胡瓜的影響評估 檢測台灣瓜類病毒病害流行情形,可了解田間主要瓜類病毒病害,蒐集不同ZYMV病毒分離株並分析其毒力與致病力,有助於在抗病篩選時使用毒力最強病毒分離株,可降低育成抗病品種抗性被打破之情形,增加品種推出壽命,並可增加相關研究資訊。瓜類種原雖多,但對其抗病毒特性描述之資料則十分缺乏,篩選結果除可供為抗病毒瓜類育成之用外,亦可增加瓜類種原之特性描述資料。抗病毒瓜類所帶有之抗性基因,極具商業價值,搭配抗病毒分子標誌之應用,並正確篩選所建立之族群具抗性基因後代,提高抗病育種之正確性。因此本計畫將執行: 一、蒐集瓜類種原,並以可鑑別抗ZYMV抗病性的分子標誌進行篩選。 二、田間病害流行調查並蒐集ZYMV病毒分離株。 應用組織培養技術大量繁殖仙履蘭及山胡椒種苗之研究 本計畫為研究仙履蘭側芽培養及山胡椒雌株組織培養技術,以期建立種苗大量繁殖技術,促進仙履蘭及山胡椒產業發展。 苗栗地區特色作物之微體繁殖 芋頭是苗栗地區重要的地方特色作物,104年栽培面積576公頃,全臺排名第二 。芋頭生育期長達8個月以上,其間易受病蟲害為害而影響產量、品質及增加栽培成 本。當中以軟腐病之影響最大,常造成球莖腐爛,影響收益甚鉅,惟目前尚無推薦之防治藥劑。帶病種苗為該病害擴散的原因之一,因此生產健康種苗將可降低感染原減少病害發生,利用組織培養繁殖技術可有效且大量繁殖健康種苗,本計畫利用 不同植物生長調節劑種類及濃度探討其對主要栽培種檳榔心芋頂芽及側芽芽體誘導及增殖之影響,以初步建立適宜之培養基條件。 十字花科種子品種純度鑑定技術之開發與應用 十字花科蔬菜富含營養價值,具產業規模,為育種研究的重點項目。種子純度影響種苗業者的聲譽,因此相關的檢定分析成為品質管理的一環。在雜交種子純度的研究包括SSR應用在甘藍品種的檢定。本計畫擬建立低成本萃取分析技術,以提供種苗業者參考運用,並開發EST-SSR分子標誌在十字花科種子純度檢定分析,提高純度檢測的準確度,及利用Multiplex PCR技術提升分析效率。 應用分子生物技術發展國產高油酸花生 花生為重要油料作物之一,種子中含有將近50%油脂,其中以油酸及亞油酸最為豐富,佔約80%左右。花生油品質取決於油脂含量及成份,而油酸/亞油酸(O/L)含量比例即為其中重要因子,通常一般花生O/L約0.9~1.2左右,高O/L比例將有利增加花生產品保存其期限。影響花生O/L的主要基因(ahFAD2A 、 ahFAD2B),最近已被了解。本實驗即要運用此標誌基因之分子生物技術輔助,大幅提升灣高油酸花生育種效率。 水稻味度基因座定位與分子標誌輔助回交選種 水稻臺南16號為近年來臺灣所育成之光不敏感性之越光近同源系,臺南16號具有與越光相似之食味品質,且具有光不敏感/晚抽穗之特性,使其適合台灣兩期作下之栽培環境。於臺南16號育成過程中所衍伸回交近同源系中,品系87-233則具有低於其他品系之食味品質,藉由87個具多型性分子標誌分析結果顯示,位於第六條染色體上臂上之臺農67號所屬染色體片段(qGCR6)為造成87-233具低食味品質之主因。本實驗擬以兩越光近同源系(87-155與87-233)雜交所衍伸之F2:3與F2:4進行qGCR6基因座定位,並應用於水稻分子標誌輔助選種過程。 南瓜及絲瓜之SSR標誌建立及品種鑑定應用 品種權與品種保護是世界潮流與共識,新品種的育成需花費相當人力與物力,若能妥善加以保護,不僅能提升產業的競爭力,也可以增加研發人員的研發動能。臺灣在作物育種能量上一直有相當優良的表現,這是臺灣農業競爭的優勢,若能擁有穩定、可信的分子標誌供品種鑑別,未來對品種的保護與應用影響深遠。本研究藉由分析探討作物簡單重複序列(simple sequence repeat, SSR),設計各種SSR基因座外的表留性性引子(primer),利用PCR反應增殖出各種SSR基因的長度,藉由SSR基因的遺傳背景不同,所造成上述間隔區長度之多型性,即可得到有用的分子標誌,藉此來建立南瓜及絲瓜之分子標誌。 萬代蘭類組織培養及與蝴蝶蘭之屬間雜交技術開發 臺灣位處熱帶及亞熱帶地區,並鄰近日本及中國等亞太市場,且因暖化所造成之環境變遷,亦提供未來臺灣發展耐高溫作物的有利條件。萬代蘭類是臺灣極具外銷潛力的新興熱帶花卉,具有許多適合發展的契機;不過,目前栽培品種以外國品種居多,種苗成本較高,再者,國內花農多已自行選育優良品系以改進進口品種適應性不良及品質不一的問題,故發展萬代蘭類種苗的生產技術實屬必要。因此,本計畫擬針對國內已商業栽培之萬代蘭及其他與萬代蘭雜交所產生的雜交屬品種,包括萬代蘭、千代蘭、Aranda及莫氏蘭等萬代蘭類,利用未花器分化的花芽進行組織培養種苗生產技術之開發,在不損及繁殖母株的條件下,企圖解決進口種苗的運輸傷害與病毒傳播問題,確保品種的純正性與品質的穩定度,及降低造成花朵無法正常開展之劣質種苗的發生機率。此外,蝴蝶蘭是臺灣重要的花卉產業,每年外銷產值已經超過30億。近年來,蝴蝶蘭的高經濟價值的特性,已經吸引荷蘭、德國及中國大陸的重視。然而,荷蘭生產設施自動化的程度高,而中國大陸或東南亞具有勞力低廉的優勢,因此就目前的蝴蝶蘭生產成本而言,臺灣很難在價格上與荷蘭與中國大陸及東南亞等國家競爭,因此能否掌握品種是臺灣能否繼續發展蝴蝶蘭產業的重要關鍵。所以,本計畫也將建立蝴蝶蘭屬與萬代蘭屬的屬間雜交技術,期能創造出特殊花色(橘色及藍色)的蝴蝶蘭屬間雜交新品種。 作物特定性狀分子標誌之建立與應用-番茄抗頸腐根腐病分子標誌建立與應用 本研究在開發番茄冠腐病(Fusarium Crown and Root Rot)抗病基因Frl SCAR分子標誌及優化PCR檢測條件一套,技轉育種公司,協助育種者早期篩選抗病基因,縮短育種世代,加速抗病優良品種育成,提升國產品種國際競爭力。 補益類中藥及食用竹類作物種苗微體繁殖技術之開發與改進 一、建立葛根、山奈兩種中藥植株開發組織培養技術以量化繁殖種苗,推廣予農民轉作栽培,期建立台灣在地好品質中藥材生產來源。 二、利用組織培養繁殖技術針對食用竹類建立無病毒健康種苗,預期可提高無病毒種苗之繁殖速率,降低種苗繁殖成本,提供農民無病毒種苗來源之新選擇,若順利推廣竹園更新使用無病毒種苗,預期可提高產量達20%以上。
英文摘要 Exploration of plant tissue culture technology and its application ■Exploration of Vanda tissue culture and rooting of in vitro shoots of micropropagation difficultyPhalaenopsis The aims of this project are to explore tissue culture and mass production of plantlets for Vanda orchids and improving the efficiency of in vitro shoot multiplication and plantlet regeneration of Phalaenopsis hybrids. Youg inflorescence and shoot tips will serve as explant source to test for optimized culture medium and methods for mass production of in vitro plantlets of Vanda orchids. The goals are to produce good quality and stable flask plantlets. Certain cultivars show difficulty ofin vitrorooting, which may lead to slow growth or decreased plantlet uniformity.In vitroshoots of both Vanda hybrids, such as Vanda Simirun x Vanda coerulea and V.Pachara Delight and Phalaenopsis Chingruey's Goldstaff ‘Gold-1’ will be used to explore the rooting on different growth regulator combinations and LED light source in order to increase growth characteristics of young plants and the overall quality. ■Establishment of micropropagation protocols and clonal propagation of selected superior hybrid individuals in Paphiopedilum Paphiopedilum has been a favorite consumer product due to its attractive color and distinctive shapes of flowers, and has reasonably potential to make it viable large-scale commercial industry. Since, diversified excellent varieties and well-established systems of micropropagation have become the main driving force in the development of Taiwan’s phalaenopsis industry. The breakthrough of the paphiopedilum orchid industry in Taiwan is also expected to rely on the breeding of new cultivars and efficient micropropagation techniques, being the one of the important limitations of Paphiopedilum orchid industry in Taiwan. The objectives of the project are to develop the efficient plant micropropagation systems, and to establish the optimal protocols for excellent individuals with novel and desirable characteristics by intrageneric crossings created by our own breeding program in Paphiopedilum. Hopefully results conducted in this project will be valuable for increasing the capacity of mass plantlet production, for reaching the demands of global markets both in quality and in quantity, and for promoting the development ofPaphiopedilumindustry in Taiwan. ■Development on healthy plantlet production of tropical ornamental aquaplants Bucephalandra sp. and Anubias barteriare foreground aquatic plants with high value in international market and possess the potential for export market development. However, export quarantine and stable production limit the development of aquatic plant industry. We applied the two-year plan for establishing in vitro plant regeneration pathway not only for mass production of healthy plantlets of Bucephalandra sp. and A. barteri , but also simplification of the quarantine and transport process. In our preliminary experiment, we have established the in vitro culture of A. barteri var. nana‘Petite’. After the removal of alga from whole plant with glutaraldehyde, we used rhizome as explants and cultured on the 1/2 MS basal medium containing 2 mg L-1 BA and 0.5 mg L-1 NAA. The average shoot formation number is 13.7 after 4 weeks of culture under light and the shoots were subcultured on 1/2 MS basal medium for rooting. We expect that our study can be applied on the mass production of healthy plantlets for improvement of aquatic plant industry. ■A technique protocol for healthy and rapid mericlonal micropropagation, and in vitro bulb production in new tuberose cultivars A technique protocol for virus analysis, detection and elimination by meristem culture or somatic embryogenesis regeneration system in new tuberose cultivars were described. By the tissue culture and its proliferation stage, follow with established for the virus detection technology, and accurate identification of healthy seedlings were conducted. Moreover, we hope to evaluate the key integration technique of virus-free plantlets production for tuberose industry sustainable application. ■In vitro propagation of Haworthia succulent plants In this project the in vitro seedlings and mericlonal propagation system of Succulent plants will be established through tissue culture methods. Various levels in maturity of the leaves, seeds and adventitious buds would be sterilized and assayed for their suitability as the material to establish the sterile propagation system of Succulent plants. By adding plant growth regulators such as BA, Kinetin, TDZ, NAA etc.to the tested basic medium the materials mentioned above are promoted and induced to regenerate whole plant through organogenesis or embryogenesis process. These seedlings and mericlonal propagation system established in Succulent plants will serve as a micropropagation platform with an increased efficiency in time duration and regeneration process for Succulent plants. In addition to serve as a strategy for free trade in domestic market, the in vitro propagation system established by this project can also be served as a solid foundation for Succulent plants with an emphasis on its regeneration and propagation efficiency for the ever growing market in both domestic and foreign country for Succulent plants. Evaluation of the response of resistant cucumber to pathogenicity differentiation ofZucchini yellow mosaic virus isolates determinated by marker-assisted selection To find out the prevlant virus infected cucurbits in the field could be achieved by surveying the epidemics of cucurbit viruses. And collection and analysis of virulence and pathogenicity of different ZYMV isolates will help us to use the highest virulence ZYMV isolate. Lower possibility of breakdown of ZYMV resistant cultivar by using the highest virulence ZYMV isolates in disease screening experiments. This method will promote the longevity of new virus resistant cucurbit cultivars and also facilitate the related research information.We will obtain the virus resistant cucurbit varieties through resistant screening and the result is not only for breeding virus resistant cucurbits but also for characterizing the cucurbit germplasm which we used. Application of virus resistant molecular marker and resistant screening could help us to screen the progeny of crossing with resistant lines and susceptible lines, and promote the validity of resistance breeding. This projec will be carried out the following items: 1.Collecting different cucurbit lines or cultigens, and checking the ZYMV resistance in seedling stage by using the ZYMV resistant gene related molecular marker. 2.Collecting the various ZYMV isolates and investigating their divergence of pathogenicity and virulence to each kind of cucurbits Study on application of tissue culture for mass propagation in Paphiopedilum and Litsea cubeba In order to establish tissue culture technology on Paphiopedilum spp. and Litsea cubeba, the project will study mass propagation seeding technology than promote the development of Paph. spp. and Litsea cubeba. Microprogation for the feature crops in Maioli area Taro is one of the important feature crops in Miaoli region. The cultivation area is around 576 hectare and ranked second in Taiwan in 2015. The cultivation period is over eight months and easily being damage by insects and diseases resulted in yield and quality failure and cost increase in this area. Soft rot on taro could make bulb rotten and is the largest among the diseases that affect farmer profit serious but no chemical could cure presently. Therefore, produce healthy seedlings or plantlets could reduce soft rot spreading and mass product effectively by using tissue culture technology. This study will focus on the effect of different plant regulators and concentrations on the bud side bulb induction and multiplication of taro (Colocasia esculenta L. var esculenta) to establish proper culture condition preliminary. Development and Application of Seed Purity Testing and Variety Identification on Brassicaceae Brassicaceae family of vegetables is full of nutrient value. Due to its scale of seed market, the breeding research in Brassicaceae is an important realm. The hybrid seed quality especially genetic purity is a critical factor influencing Brassicaceae family production and reputation of the plant seed enterprise. Studies on hybrid seed purity include SSR applications in Brassicaceae family cultivars has been developed. The aim of this project is to establish a low-cost extraction and analysis technique to provide for plant seed enterprises. Furthermore, to develop a rapid and accurate molecular identification method such as EST-SSR marker and Multiplex PCR technique for testing the genetic purity of Brassicaceae seeds. Breeding high oleic and linoleic fatty acid peanut by marker assisted selection. Peanut is an oilseed crop, the seeds contain about 50% of oil and oleic and linoleic fatty acids comprise accounted for about 80% . The quality of peanut oil depends on the properties of the oil, such as the oleic/linoleic acid ratio (O/L ratio). In general the O/L ratio among peanut cultivars varied between 0.9~1.2. A high O/L ratio can increase the shelf life of peanut products. The two genes related to the O/L ratio, ahFAD2A and ahFAD2B, were previously identified and reported in peanut. In this project, we tried to use the marker genes of the molecular biotechnology breeding to get a high O/L ratio peanut cultivar. Fine mapping of a gene for highly glossiness of cooked rice, qGCR6 in japonica rice variety, Koshihikari Tainan 16 possessed the excellent eating quality of japonica rice variety, Koshihikari, but without photoperiod sensitivity. In the currently study, two BILs 87-155 and 87-233 derived from between TNG67 and Koshihikari showed significantly different in glossiness of cooked rice (GCR) determined by Toyo-taste meter. Genetic map based on the genotype of 87 polymorphic SSR markers indicated one chromosome segment, qGCR6 locus on the short arm of chromosome 6 is different between two lines. To understand the genetic character, two BILs 87-155 and 87-233 will cross to create a mapping population in 2016. Fine genetic mapping of qGCR6 would carry out in 2017. The knowledge from this result might applied to improve the eating quality of japonica rice in the further. The Development of SSR Markers and Cultivars Identification for Pumpkin and Luffa Crop breeding is very important for the development of agriculture. It will cost much investment not only labor but also money. Therefore, cultivars right and protection recently have been to be popular common view in the world. The ability of crop breeding is very powerful in Taiwan. It is to be the competitive advantage in agriculture. Therefore, a stable, efficiency molecular marker system is very important for agricultural development, since DNA marker can offer the protection of cultivars right. In the study, we will develop molecular markers dervied from simple sequence repeat (SSR) for pumpkin and luffa. The length polymorphism of SSR loci can be shown after PCR amplification for different varieties. The development of tissue culture in Vanda and its allied genera and the intergeneric hybridization between Vanda and Phalaenopsis Due to Taiwan being situated in a tropical and subtropical region, and neighboring Japan and China and other Asia-Pacific markets, and because of environmental changes caused by global warming, its unique conditions offer advantages for the future development of high temperature resistant crops. Vanda alliances are emerging tropical flowers, with great export potential in Taiwan. However, the current cultivars planted are mostly from foreign countries, which seedlings are at higher cost; moreover, most domestic growers have been breeding new cultivars to improve the poor adaptability and inconsistent quality of imported cultivars, so the development of tissue culture techniques for Vanda alliances seedling propagation are necessary. Therefore, this project is intended for the development of tissue culture techniques for Vanda alliances, including Vanda, Ascocenda, Aranda, Mokara, and other genera hybridized with Vanda those have been commercially planted in Taiwan, seedling propagation by flora bud which has not differentiated into floral organs yet. Attempt to solve the transportation damage and virus transmission of imported seedlings, ensure the cultivar correctness and the stability of seedling qualities and reduce the probability of production of inferior seedlings that can not bloom normally. In addition, moth orchid is very popular and important orchids in Taiwan. According to the inspection, the exportation value of moth orchids in Taiwan is over 3.0 billions NT dollars. Hence, moth orchid is considered as a highly valuable crops based on the economical viewpoint of market. Recently, the stringent competition of orchids market from Europe and Mainland China/South Asia has been under running. Since the production investment in Taiwan is higher than Europe and Mainland China as a result of the successful automatical culture system in Europe and low labor investment in Mainland China/South Asia. Therefore, the orchid varieties are the most key point for the successful competition for Taiwan. In the present study, we also intend to obtain intergeneric orchids with unique floral color through the crossing between Phalaenopsis and Vanda based on intergeneric hybrida. The establishment and application of specific molecular markers for Fusarium crown and root rot resistance gene in tomato. In this study, SCAR markers for the resistance genes Frl in tomato Fusarium Crown and Root will be developed and optimized a set of PCR detection condition. To enhance the international competitivenes of domestic varieties, This technology will transfer to seed company for early screening resistant materials, shortening breeding generations, and accelerating breeding disease resistant varieties. Study on in vitro micropropagation of traditional Chinese medicines and edible bamboo crops 一、We try to develop in vitro micropropagation techniques of Chinese medicine crop(Pueraria spp, Kaempferia galanga L.), providing good quality Chinese herbal medicine sources, in the future. 二、The establishment of healthy virus-free bamboo seedlings, is expected to improve the rate of virus-free seedling propagation, reduce seedling reproductive costs. And if the farmers use virus-free seedlings, is expected to increase yield by 20% or more.
其他研究人員 黃柄龍 陳國憲 丁昭伶 任珮君 周建銘 蔡文錫 陳福旗 張珈錡 沈榮壽 宋品慧 黃光亮 高建元 李淑真 施純堅 王聖善 周明燕 陳榮坤 陳麗如 朱玉 徐善德 翁一司 吳靜霞 廖玉珠 林子凱
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